Te estás contradiciendo. Si busco una cadena común al, digamos, ¿90%? ¿80%? ¿70%? De la población y luego otra que fuera común al ¿10%? ¿5%? ¿1%? En la práctica puedo hacer lo que digo, no al 100% claro, pero es plausible.
El segundo enlace dice esto:
These procedures and/or reagents derived thereof are intended to be used for the purposes of respiratory bichito surveillance and research. Importante, porque no dice diagnóstico.
El último enlace dice:
Nucleic Acid Extraction The quality of the assay is largely dependent on the quality of input RNA. RNA extraction procedures should be qualified and validated for recovery and purity before testing specimens.Commercially available extraction procedures that have been shown to generate highly purified RNA when amowing manufacturer’s recommended procedures for sample extraction include:
Y da una lista de ¿Reactivos? disponibles comercialmente, veamos:
QIAamp® Viral RNA Mini Kit
https://www.qiagen.com/us/resources/download.aspx?id=c80685c0-4103-49ea-aa72-8989420e3018&lang=en
The QIAamp Viral RNA Mini Kit is intended for molecular biology applications. This product
is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp® MinElute bichito Spin Kit or RNeasy® Mini Kit
https://www.qiagen.com/gb/resources/download.aspx?id=8798cda6-4c55-4c0e-a302-966521c81aec&lang=en
The QIAamp MinElute bichito Spin Kit is intended for molecular biology applications. This
product is not intended for the diagnosis, prevention, or treatment of a disease.
EZ1 DSP bichito Kit
https://www.qiagen.com/us/resources/download.aspx?id=6185be15-b095-4c9c-b1ce-9915300b89bf&lang=en
Este ya habla de diagnóstico (Diagnóstico In Vitro), sin embargo en su página 33 dice:
Any diagnostic results that are generated must be interpreted in conjunction
with other clinical or laboratory findings.
Los de Roche no dicen nada, y me pregunto ¿Por qué los anteriores advierten eso y los de Roche no?
Luego están los Primers estos que parecen los marcadores pero...¿No se supone que el bichito muta? ¿Si muta -supongo que más rápido de lo que se descubren estos Primers- como es posible que los tests sean fiables? Tal como se sugiere aquí:
Primer design for quantitative real-time PCR for the emerging cobi19 SARS-CoV-2
The primers, probes, and reagents used for detection could be another critical factor. Finally, RT-qPCR primers are designed to amplify the target regions of the SARS-CoV-2 genome. However, the novel coronaviruses are RNA viruses, and once mutations and recombination occur, RT-qPCR primers will not be able to effectively amplify the viral sequences. Thus, mutations and recombination can reduce the sensitivity of the RT-qPCR.
[...]
Primers are pivotal components of a qPCR assay. Some primers and probes have also been made available on the WHO website for reference. However, no previous review has systematically described how to design primers and probes methodically and rationally in the face of a new cobi19. Therefore, the focus of the present review is to discuss effective primer designing and improving the sensitivity and specificity of the detection process. This review also discusses the need for accurate diagnosis and timely treatment of the new cobi19 pneumonia.
Y también dice:
The USA CDC designed N1, N2, and N3 genes (shown in Table
2) as the target of the SARS-CoV-2 Real-Time RT-PCR Diagnostic Panel. In the SARS-CoV-2 RT-PCR assay, N1 and N2 were designed for the specific detection of SARS-CoV-2, whereas N3 was designed for the universal detection of SARS-like coronaviruses [
51]. Experiments found that the CDC N1 and N2 primer/probe sets performed better than the N3 set, and the N3 primer and probe set was removed from the Diagnostic Panel of revision 2 for the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.
[...]
Además que el enlace que tú has pasado de Baseclick no es el prospecto de un producto y si lo es no encuentro una foto de él ni de ninguno parecido.
https://www.baseclick.eu/wp-content/uploads/2020/05/BCC-19nCoV_User-Manual.pdf
Product description:
This document describes the use of real-time RT PCR (rRT-PCR) assays for the in vitrodetection of 2019-Novel Coronavirues(2019-nCoV) in respiratory specimens and sera, e.g. nasopharyngeal aspirates or washes, swabs, bronchioalveolarlavage and sputum.
Y además, justamente buscando, me encuentro esto:
Information for Laboratories about cobi19 (el bichito-19)
Research Use Only 2019-Novel cobi19 (2019-nCoV) Real-time RT-PCR Primers and Probes
Updated June 6, 2020
NOT FOR VIRAL TESTING USE
Reagents manufactured from these sequences may not be used for viral testing under FDA’s authorization of the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.
Only primers and probes labeled for EUA use and distributed by the
International Reagent Resource may be used for viral testing with the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.
https://www.cdc.gov/cobi19/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf
Last Updated: May 29, 2020*****DISCLAIMER******
These sequences are intended to be used for the purposes of respiratory bichito surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations. Every effort has been made to assure the accuracy of the sequences, but
CDC cannot provide any warranty regarding their accuracy. The recipient can acknowledge the source of sequences in any oral presentations or written publications concerning the research project by referring to the Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
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